Host influence on the characteristics of Venezuelan equine encephalomyelitis virus.
نویسندگان
چکیده
Heydrick, Fred P. (Fort Detrick, Frederick, Md.), Ralph F. Wachter, and Henry J. Hearn, Jr. Host influence on the characteristics of Venezuelan equine encephalomyelitis virus. J. Bacteriol. 91:2343-2348. 1966.-Alterations in plaque size, virulence, and lipid content of Venezuelan equine encephalomyelitis (VEE) virus were examined for possible interrelationships among these properties during 10 serial passages in embryonated eggs, suckling mice, chick embryo fibroblasts, and L cells. The chick embryo host maintained the same large-plaque and virulence properties of the virus through 10 passages as seen in the original seed. Passage of virus in either L cells or chick fibroblasts rapidly produced populations that were, in the main, intermediate with respect to plaque size and virulence. Passage of virus in suckling mouse brain yielded populations that were intermediate with respect to plaque size only. The nature of the lipid of the virus, in terms of the ratio of petroleum ether-soluble to -insoluble lipid, changed after only one passage in all systems except in chick embryos. Nine additional serial passages failed to enhance these changes in viral lipid, suggesting that the decrease in the large-plaque and virulence properties was not directly associated with changes in lipid content.
منابع مشابه
Eastern, Western and Venezuelan Equine Encephalomyelitis Sleeping Sickness Eastern Equine Encephalomyelitis (EEE), Eastern Equine Encephalitis, Eastern Encephalitis Western Equine Encephalomyelitis (WEE), Western Equine Encephalitis Venezuelan Equine Encephalomyelitis (VEE), Peste Loca, Venezuelan Equine Encephalitis, Venezuelan Encephalitis, Venezuelan Equine Fever
Eastern equine encephalomyelitis (EEE), western equine encephalomyelitis (WEE), and Venezuelan equine encephalomyelitis (VEE) viruses are mosquito-borne pathogens that can cause nonspecific illnesses and encephalitis in equids (horses, mules, burros, donkeys and zebras) and humans in the Americas. Some of these viruses also affect birds and occasionally other mammals. No specific treatment is a...
متن کاملPlaque size and virulence of attenuated Venezuelan equine encephalomyelitis virus after passage in various hosts.
متن کامل
Cell surface antigen induced by venezuelan equine encephalomyelitis virus.
By immunofluorescence staining, a specific surface antigen induced by Venezuelan equine encephalomyelitis virus was detected on L-929 cells. Formation of the antigen was independent of viral ribonucleic acid synthesis.
متن کاملChromatography of Venezuelan equine encephalomyelitis virus strains on calcium phosphate.
Column chromatography of selected Venezuelan equine encephalomyelitis (VEE) viruses on calcium phosphate gel offered a simple and reproducible method for examination of biochemical characteristics and relatedness of strains within the VEE complex. Members of antigenic subgroup I demonstrated a series of elution profiles within a narrow range of 0.22 to 0.25 M phosphate buffer. Members of antige...
متن کاملNonviable Venezuelan equine encephalomyelitis hemagglutinin prepared from tissue cultures by gamma radiation.
Hemagglutinins of Venezuelan equine encephalomyelitis virus were produced in tissue cultures, and the infective virus was rendered nonviable by exposure to gamma radiation.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of bacteriology
دوره 91 6 شماره
صفحات -
تاریخ انتشار 1966